Journal: Immunity, Inflammation and Disease

Article Title: Inhibition of TMUB1 blocks apoptosis and NF‐κB pathway‐mediated inflammation in recurrent spontaneous abortion

doi: 10.1002/iid3.879

Figure Lengend Snippet: Effects of LPS on apoptosis and inflammation in placental tissues of pregnant mice (A) Representative images of TUNEL staining at magnification X200 (Scale bars: 100 µm) and magnification X400 (Scale bars: 50 µm). (B) The mRNA levels of IL‐6 and TNF‐α were measured with quantitative RT‐PCR. (C) The content of IL‐6 and TNF‐α was quantified by ELISA. (D) Western blot analysis for p‐p65 and p65 (left), and the quantification of the p‐p65/p65 ratio (right). ELISA, enzyme‐linked immunosorbent assay; IL‐6, interleukin‐6; LPS, lipopolysaccharide; mRNA, messenger RNA; RT‐PCR, real‐time polymerase chain reaction; TNF‐α, tumor necrosis factor‐α; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.

Article Snippet: The content of IL‐6 and TNF‐α was measured using the Mouse TNF‐α ELISA Kit, Mouse IL‐6 ELISA Kit, Human TNF‐α ELISA Kit, and Human IL‐6 ELISA Kit (LIANKE Biotech) according to the manufacturer's instructions.

Techniques: TUNEL Assay, Staining, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Journal: Immunity, Inflammation and Disease

Article Title: Inhibition of TMUB1 blocks apoptosis and NF‐κB pathway‐mediated inflammation in recurrent spontaneous abortion

doi: 10.1002/iid3.879

Figure Lengend Snippet: Effects of TMUB1 silencing on inflammation in LPS‐induced trophoblast cells. (B) The levels of IL‐6 and TNF‐α in the different treatments were measured by quantitative RT‐PCR and ELISA. (B) The expression of p‐IKKα/β and IKKα was detected by western blot, and the ratio of p‐IKKα/β/IKKα was quantified in different groups. (C) The expression of p‐p65 and p65 was detected by western blot, and the ratio of p‐p65/p65 was quantified in different groups. (D) The protein content of NF‐κB p65 in the cytoplasm and nucleus was detected by western blot. ELISA, enzyme‐linked immunosorbent assay; IL‐6, interleukin‐6; LPS, lipopolysaccharide; RT‐PCR, real‐time polymerase chain reaction; TMUB1, transmembrane and ubiquitin‐like domain containing 1; TNF‐α, tumor necrosis factor‐α.

Article Snippet: The content of IL‐6 and TNF‐α was measured using the Mouse TNF‐α ELISA Kit, Mouse IL‐6 ELISA Kit, Human TNF‐α ELISA Kit, and Human IL‐6 ELISA Kit (LIANKE Biotech) according to the manufacturer's instructions.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Ubiquitin Proteomics

Journal: Immunity, Inflammation and Disease

Article Title: Inhibition of TMUB1 blocks apoptosis and NF‐κB pathway‐mediated inflammation in recurrent spontaneous abortion

doi: 10.1002/iid3.879

Figure Lengend Snippet: Effects of TMUB1 silencing in LPS‐induced mice. Pregnant mice were injected with lentivirus knocking down TMUB1 and then injected intraperitoneally with 0.15 μg/g LPS to induce abortion. (A) Pathological and anatomical observation of uteruses in pregnant mice. (B) The embryo absorption rate and abortion rate were calculated respectively in pregnant mice. (C) TUNEL staining was used to detect apoptosis in LPS‐induced mice with TMUB1 knockdown. Scale bar: 50 µm. (D) The mRNA levels of IL‐6 and TNF‐α were measured by quantitative RT‐PCR. (E) The protein content of p‐p65 and p65 was detected by western blot, and the ratio of p‐p65/p65 was quantified in different groups. DAPI, 4′,6‐diamidino‐2‐phenylindole; LPS, lipopolysaccharide; mRNA, messenger; RT‐PCR, real‐time polymerase chain reaction; TMUB1, transmembrane and ubiquitin‐like domain containing 1; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.

Article Snippet: The content of IL‐6 and TNF‐α was measured using the Mouse TNF‐α ELISA Kit, Mouse IL‐6 ELISA Kit, Human TNF‐α ELISA Kit, and Human IL‐6 ELISA Kit (LIANKE Biotech) according to the manufacturer's instructions.

Techniques: Injection, TUNEL Assay, Staining, Knockdown, Quantitative RT-PCR, Western Blot, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Ubiquitin Proteomics

Journal: Breast Cancer : Targets and Therapy

Article Title: The role of the chemokine receptor XCR1 in breast cancer cells

doi: 10.2147/BCTT.S126184

Figure Lengend Snippet: Expression of XCR1 in breast cancer cell lines. Notes: Expression of XCR1 mRNA was measured by quantitative RT-PCR ( A ) in 1 normal breast epithelial cells and 9 breast cancer cell lines. XCR1 protein expressions were detected by immunohistochemistry in 10 formalin-fixed, paraffin-embedded breast cancer tissue sections (5 tissue sections are ER+, the others are ER−), the expression of XCR1 protein is associated with ER expression ( B , C ). * p <0.05, ** p <0.01. Abbreviations: ER, estrogen receptor; RT-PCR, real-time polymerase chain reaction.

Article Snippet: Human breast cancer cell lines, MDA-MB-231, MDA-MB-468, ZR-75-30, T474D, MCF-7, ZR-75-30, and SK-BR-3, and one normal breast epithelial cell line, namely MCF-10A, were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Journal: Scientific Reports

Article Title: LOXL2 drives epithelial-mesenchymal transition via activation of IRE1-XBP1 signalling pathway

doi: 10.1038/srep44988

Figure Lengend Snippet: ( a ) Promoter assays were performed in control mouse Eo771 (pLKO) (left) and human MDA-MB-231 cells (shEGFP) (right) and in cells silenced for LOXL2 (shLOXL2). Error bars represent the s.e.m. (n = 3) (*p < 0.05, **p < 0.01, ***p < 0.001). ( b ) LOXL2 knockdown in Eo771 and MDA-MB-231 cells abrogates XBP1 splicing. Cells used in ( a ) were processed for RT-PCR analysis of spliced XBP1. GAPDH levels serve as loading control. Unspliced ( XBP1 u) and spliced ( XBP1s ) forms of XBP1 are indicated. (*) XBP1 hybrid band. One representative RT-PCR analysis of three independent experiments is shown.

Article Snippet: Human HEK293T, MDA-MB-231 and Hs578T, mouse Eo771 and dog MDCK-II cell lines were obtained from the American Type Culture Collection and grown in DMEM media (Gibco), supplemented with 10% foetal bovine serum, 10 mmol/L glutamine (Life Technologies) and 1% penicillin/streptomycin (Invitrogen).

Techniques: Control, Knockdown, Reverse Transcription Polymerase Chain Reaction